Developing
Propagation Protocols of Selected High
Altitude Endemic Medicinal Umbellifers of the Himalaya with a Focus on Angelica
archangelica L. var himalaica
Cl.
Supervisors:
Dr. U. Dhar, GBPIHED, Kosi-Katarmal, Almora
Prof. Y.P.S. Pangtey, D.S.B. Campus, Kumaun University, Nainital
Summary:
Considering
the importance of conservation and sustainable use of Himalayan medicinal
plants, the present work focuses on development of conventional (seed
germination and vegetative propagation) and in
vitro propagation protocols for three important Himalayan Umbellifers,
viz., Heracleum candicans, Selinum tenuifolium and Angelica archangelica.
The study describes in detail the aspects of: (i) seed germination;
(ii) vegetative
propagation through rhizome cuttings, and (iii) in-vitro propagation protocols. Seed germination chapter
describes various pretreatments for improving germination ability and
reduce mean germination time (MGT). Viability of seeds in all the cases
was generally high. Germination ability of seeds from different sources
(populations) showed variation among populations. Improvement in
germination of target species was achieved through various interventions.
Germination percentage, in all target species, improved under chilling (H.
candicans: 88.9%, 14 d; S.
tenuifolium: 86.7%, 14 d; A.
archangelica: 77.8%, 7 d). In most cases, chilling also reduced the
MGT. In case of A.
archangelica, although highest germination (77.8%) was achieved in 7 d
chilling alongwith GA3 500 ppm pretreatment, application of KNO3
(500 ppm) was also successful in improving germination (77.8%).
Considering practical feasibility and cost effectiveness, chilling
treatment was recommended as one of the best options, especially for rural
inhabitants.
Vegetative
propagation chapter describes and discusses conventional method through
vegetative propagation of rhizome cutting in two species (H. candicans and A.
archangelica). In case of H.
candicans IBA (100 ppm) was found to be the best treatment [( percent
rooting 66.7, along with maximum root number (12) and root length (13.9
cm)]. On the contrary, in A.
archangelica significantly (p< 0.05) high rooting was achieved
under control condition (75% rooting; 10.2 root number and root length
17.5cm).
In-vitro chapter details micropropagation protocols developed for the target species. Various factors responsible for explant establishment have been discussed. Sterilization of the explants was achieved using HgCl2 in case of cotyledonary leaf and node of H. candicans and petiole of A. archangelica. Whereas, Bavistin and HgCl2 were effective for mature leaves of S. tenuifolium. Organogenic protocol, using two type of explants - cotyledonary node and cotyledonary leaf- was developed in case of H. candicans. The protocol resulted in achieving higher number of shoots. 10然 BA was found best for achieving maximum shoot number and shoot length whereas, maximum rooting (80.7%) was achieved in 4然 IBA along with higher root number (5.8 root/explant). Nearly 70% survival of plantlets was observed when rooted shoots were transferred for acclimatization in soil: sand: moss (1:1:1) substratum. Comparative advantages of the present protocols have been discussed. In case of S. tenuifolium the protocol was developed through somatic embryogenesis. The best response with regard to embryogenic callus (93%) as well as plantlets formation (8.1shoot / explant) was obtained in 2然 BA and 2然 IBA after eight week of embryogenesis. Potential of production of synthetic seed is discussed. 65% plantlets survived when transferred to soil: sand: moss (1:1:1). For A. archangelica, callus induction through petiole explants (1然 2,4-D and NAA 1然) has been achieved. Also, shoots developed using seedling explants (2 然 BA and 1然 NAA). The study, therefore, provides complete and comprehensive details of propagation through various means in identified target species. The possibilities of using the results of this study for conservation and utilization of these species are discussed in detail.
Meena Joshi
Subject : Botany
Ph. D - Botany Kumaun University, Nainital
Correspondence Address:
, CBD/GBPIHED, Kosi-Katarmal, Almora,Uttaranchal-263 643
Work Title